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LABORATORIES AND QUALITY CONTROL DEPARTMENT

Introduction:

Laboratories at NCARE have been established to support the researchers with the analysis they need and to provide farmers with fundamental services to solve their agricultural problems.

Main Goals:

  1. Improving the quality and quantity of the agricultural outputs.

  2. Increasing the productivity and reducing the production cost.

  3. Achieving maximum utilization of the limited natural resource in Jordan.

  4. Improving crop competitiveness of Jordanian agricultural products in local and foreign and new markets.

To achieve the previous goals, the laboratories department is carrying out the following responsibilities:

  • Conducting laboratory analysis for research and services for different agricultural sectors.

  • Conducting laboratory experiments.

  • Training programs on Lab analysis .

  • Conducting and identifying farmer problems and providing the required assessment for solving their problem.

Staff:

M.Sc.

8

B.Sc.

12

Diploma

2

General Secondary Education

7


Laboratories at NCARE are:

  1. Soil analysis.

  2. Water analysis .

  3. Plant analysis . 

  4. Fertilizers.

  5. Plant protection .

  6. Microbiology .

  7. Tissue culture .

  8. Forage .

  9. Biotechnology.

  10. Quality Control office

Plant & Fertilizers Laboratory

Target groups:

  • Farmers.

  • Researchers.

  • Agricultural Companies.                                                                                                  

  • Ministry of Agriculture.

  • Universities.

  • Ministries and Government Departments.

  • Regional and International Organizations.

Quality control office:

From the consciousness of National Center for Agricultural Research and Extension (NCARE) to the importance of meeting the requirements and satisfying the needs of target groups by getting an accurate and precise laboratory results in physical, chemical, and biological tests for the main agricultural items (soil, plants, fertilizers, water), and also from recognition of the top management and laboratory management to the significance and importance of all test stages starting from customer inquiry or questions up to receiving the test result certificate and according to the needs of the country and citizens allowing Jordanian agricultural products to reach  European and West markets, and by supporting the recommendations for (improvement of exports project). This will encourage (NCARE)  to get a national accreditation from Jordan Institution for Standards & Metrology (JISM) in accordance to ISO/IEC 17025 requirements.

Laboratory and Quality Control Department performed and established a quality system starting from plant & fertilizer labs and implemented by which the customer samples were analyzed using special and modern equipments. In addition to that, samples were applied using standard analytical methods for existing concentrations of nutrient required for plant growth, including major nutrients (N, P, K), Secondary nutrients (Ca, Mg), micronutrients (Fe, Mn, Zn, Cu, B, Mo, Cl), and toxic elements- such as Al, Cd, Li, Na, and Pb.

Soil Analysis Laboratories:

Soil is defined as the unconsolidated mineral material on the surface of earth that has been subjected to and influenced by genetic and environmental factors of: parent material, climate (including moisture and temperature effects), macro- and micro-organisms, and topography. These factors, over a period of time, produce a type of soil, differs from original material in many physical, chemical, biological, and morphological properties and characteristics. Soil is defined most suitable place which plant can grow up and achieve it necessary materials from it.

The objectives of soil analysis are :

  • Define soil content in order to improve and solve problem.

  • Define fertility rate by which the right quantity and quality of fertilizers can be added, in order to improve soil conditions.

  • Supporting researchers by providing them by the required information.

Soil Analysis Laboratories

  Samples fees:

Soil analysis

Cost for Researchers, companies (JD)

Cost for Farmers (JD)

pH

5.00

1.50

Electric conductivity (EC)

5.00

1.50

Available Phosphorus

8.00

2.00

Available Potassium

8.00

2.00

Calcium carbonate

4.00

1.00

Texture

12.00

2.00

Organic Matter

10.00

2.50

Nitrogen

15.00

4.00

Moisture

5.00

1.00

Cation Exchange capacity

12.00

4.00

Gypsum

8.00

2.00

Bulk Density

6.00

1.50

Nitrate

15.00

4.00

Trace element for each

10.00

3.00

Heavy metals for each

15.00

4.00

 Cations and Anions

Ca

4.00

0.75

Mg

4.00

0.75

Na

8.00

1.25

K

8.00

1.25

Cl

4.00

0.75

CO3

4.00

0.75

HCO3

4.00

0.75

Others

15.00

4.00

 

Water Analysis Laboratories:

Jordan is considered a poor water resources country; this creates the need for a wise water strategy.

Water analysis lab mission is to analyze water samples for agricultural objectives and determine its utility for agricultural purposes. Many years ago, we started using treated wastewater in agricultural research to find out its utility for irrigating certain crops.

Samples fees:

Water analysis

Cost for Researchers, companies (JD)

Cost for Farmers (JD)

pH

3.00

0.75

Electric conductivity (EC)

3.00

0.75

Phosphorus

8.00

2.00

Nitrate

8.00

2.00

TDS

8.00

2.00

TSS

7.00

1.50

Trace element for each

10.00

3.00

Heavy metals for each

15.00

4.00

 Cations and Anions

Ca

4.00

0.75

Mg

4.00

0.75

Na

8.00

1.25

K

8.00

1.25

Cl

4.00

0.75

CO3

4.00

0.75

HCO3

4.00

0.75

Others

8.00

2.00

 

Water Laboratory

 

Plants Analysis Laboratory:

Healthy plants contain predictable concentrations of essential elements. Growers, who submit plant tissue samples for analysis, can monitor actual levels of these elements within a crop throughout the season. They can find out deficiencies or toxicities which help in optimizing fertilization in timely manner.

Plant Laboratory analyzes plant tissue samples chemically. The analysis measures the existing concentrations of nutrient required  for plant growth, including: major nutrients (N, P, K), secondary nutrients (Ca, Mg), micronutrients (Fe, Mn, Zn, Cu, B, Mo, Cl), and toxic elements such as Al, Cd, Li, Na, and Pb.

Plant analysis reports provide voluble data which is necessary for making management decisions. Growers can reduce application rates and fine tune fertilizer programs. Compiling yield data with plant reports provides essential records of nutrient used in waste management systems. Plant analysis reports can serve as voluble evidence of responsible crop management and environmental stewardship.

Plants Analysis Laboratory

 

Samples fees:

Plant analysis

Cost for Researchers, Companies (JD)

Cost for Farmers (JD)

Total Nitrogen (N )

15.00

4.00

Phosphorus (P).

10.00

2.50

Potassium (K).

10.00

2.00

Calcium (Ca)

10.00

3.00

Magnesium (Mg)

10.00

3.00

Chlore (Cl)

5.00

1.50

Manganese (Mn)

10.00

3.00

Sodium (Na)

10.00

3.00

Iron (Fe)

10.00

3.00

Copper (Cu)

10.00

3.00

Zinc (Zn)

10.00

3.00

Molybdenum (Mo)

15.00

4.00

Ash

10.00

2.00

Moisture

5.00

1.00

Nitrate (NO3)

15.00

4.00

Heavy Metals

15.00

4.00

Others

15.00

4.00

 

Fertilizers Analysis Laboratory:

Fertilizer is simply the plant food; just like human body needs vitamins and minerals, plants need nutrients in order to grow.

Fertilizer is the most controllable source of plant nutrients, so careful selection of nutrients rates, placement, and timing of fertilizers placement, will enable farmers to supply the food plants needed at nearly optimum levels, to achieve economic and environmental efficiency.

Fertilizers Lab. is responsible for the analysis of imported or locally manufactured fertilizers, to verify the accuracy of sample analysis results and meet regulator specifications. 

Fertilizers Analysis Laboratory

 

Samples fees:

Fertilizers analysis

Cost for Researchers, companies (JD)

Cost for Farmers (JD)

pH

4.00

1.00

Electric conductivity (EC)

4.00

1.00

Total Nitrogen (N %)

15.00

4.00

Phosphorus (P2O5).

10.00

2.50

Potassium (K2O).

10.00

2.00

Calcium (CaO)

10.00

3.00

Magnesium (MgO)

10.00

3.00

Chlore (Cl)

5.00

1.50

Manganese (Mn)

10.00

3.00

Sodium (Na)

10.00

3.00

Iron (Fe)

10.00

3.00

Copper (Cu)

10.00

3.00

Zinc (Zn)

10.00

3.00

Molybdenum (Mo)

15.00

4.00

Organic Metter (O M)

10.00

2.50

Ash

10.00

2.00

Moisture

5.00

1.00

Bulk Density

5.00

1.00

Heavy Metals

15.00

4.00

Others

15.00

4.00


Plant Protection Laboratories:

Plant protection is usually play a major role of high quality and quantities of agricultural products and crops without disturbing and polluting the agro- ecosystem, diagnosis of most plant diseases and pests usually occurs in laboratories to determine the causal agent and suggest a proper management control program.

 Plant protection laboratories are available NCARE and most of them are able to identify and diagnose plant pests and diseases on different kinds of crops and fruit trees. Infected samples were  sent to laboratories from Farmers, quarantine borders centers, governorates agriculture departments and also from NCARE researchers.

Also many training programs were held in plant protection laboratories for university students and agricultural engineers in the fields of diagnosis of plant pests and using laboratories facilities.

Main Objectives:

Carry out laboratory analysis to soil, plants, tubers and other planting materials for diseases and pests diagnosis for farmers, agricultural companies and for agricultural quarantine samples .However, plant protection labs.are conducting laboratory analysis to plant protection researches.

Laboratories of plant protection include the fallowing:

1. Virology laboratory:

 This lab. equipped with high tech. equipments and instruments for viral diseases detection and identification using serological tests (ELISA).

Plant Virology laboratory

 

2. Mycology & Bacteriology laboratory:

The main duties of this lab is to identify  fungi, bacteria which infect wide range of plants, and isolate these causal agents from soil, plant tissues, seeds and any other planting material using biochemical tests and make isolation on different types of growth media.

Mycology & Bacteriology laboratory

3. Nematology laboratory:

Identify samples (Plant, soil) received from farmers and guide them to suitable managements control program to reduce the losses cased by nematode infection.

4. Laboratories in regional centers:

a)  Jordan valley laboratories

  • Provide plant diseases diagnostic services to farmers in Jordan valley and identify casual agents attack their crops.

  • Analyze soil, plant samples for research purposes.

  • Conduct field visits to farms and solve plant pathogenic problems.

Contain three main labs.

  • Plant Mycology & Bacteriology: plant pathogens diagnosis from soil, plant, and seed samples.

  • Insect laboratory: identification and classification of insect that cause crop losses.

b)  Rabba laboratory.consists of two main laboratories:

  • Entomology lab: identification of key insects which attack, field crops, fruit trees, vegetables in the region and guide the farmer with proper control measurements to combat insect pests and reduce their losses.

  • Mycology lab: isolate main fungal diseases which attack fruit trees &field crops in the area on growth media and prescribe effective control measurements to farmers.

Samples fees:

Plant Protection

Farmers (JD)

Researchers & Companies

Fungi

1.50

15.00

Bacteria

10.00

30.00

Potato viruses

40.0

180.0

Vegetable viruses

3.0

18.0

Fruit trees viruses

3.0

20.0

Nematodes

3.0

12

Microbiology Laboratory

The main objectives are:

  1. Determination the degree of bacterial contamination and its effect on water, soil and human health.

  2. Studying the effect of this contamination on plant production types and agriculture.

  3. Studying the effect of this contamination on human health.

  4. Facilitate usage of treated wastewater in irrigation.

  5. Indicating the importance of methods in minimizing the contamination.

Procedures:

Collecting samples in sterile conditions and sending them directly to the lab in icebox.

Analyzing samples using standard methods. 

Microbiology laboratory

Samples fees:

Microbiology

Researchers & Companies (JD)

Farmers (JD)

Total and fecal Coliform (water)

25.00

10.00

Total and fecal Coliform (plant or soil)

40.00

15.00

Nematode eggs

20.00

8.00

Biologicl Oxygen Demand (BOD)

20.00

8.00

Chimical Oxygen Demand (COD)

20.00

8.00

Bacterial total count

10.00

5.00

Laboratory of Plant Tissue Culture:

Plant tissue culture lab established in 1990 as apart  of plant production department at MOA, the lab started working research on micro propagation of apple and developed research to work on other crops, such as palm date, potato, grape, citrus and some medicinal and ornamental plants. In 1999, it was join to NCARE.

Advantages of plant tissue culture: 

Tissue Culture Laboratory

  • Large number of plants is produced in a small space compared to the traditional ways.

  • Preservation of the genetic resources of plants.

  • Production of true-to-type plants, identical to mother plant.

  • Plants produced are disease-free, especially from viral diseases.

  • Make use of somaclonal variation.

  • Inducing some mutations to produce superior plants that are disease-resistant.

  • Genetic engineering for species to produce genetically modified plants.

Forage Analysis Laboratory:

Forage laboratory aims to achieve more beneficial results in animal production sector through:

Preparing formulations of the least cost ration to supply the animals with requirements.
Determining high quality product that can affect animal health in association with integrated animal production program by conducting analysis of feed rations samples to evaluate different treatments and their effect.

Samples fees:

Forage

Cost For Researchers, or Companies (JD)

Protein

15.00

Phosphorus (P).

10.00

Potassium (K).

10.00

Calcium (Ca)

10.00

Magnesium (Mg)

10.00

Fat

15.00

Manganese (Mn)

10.00

Sodium (Na)

10.00

Gross energy

15.00

Iron (Fe)

10.00

Copper (Cu)

10.00

Zinc (Zn)

10.00

Molybdenum (Mo)

15.00

Ash

10.00

Moisture

5.00

Fiber

15.00

Heavy Metals

15.00

 

BIOTECHNOLOGY

 

 Biotechnology includes the use of techniques for the improvement of the characteristics of economically important plants and animals and for the development of micro-organisms to act on the environment. The biotechnology lab is an important lab at NCARE, conducting applied research in the area of plant production. The lab also provides service to the local societies and framers. The lab staff solve the farmer problem through detecting the genetic variations among different plant crops.

Set up goal of the lab:

To use the DNA fingerprinting techniques for studying the genetic variations of wild, cultivated and imported crops and use of the biotechnology methods for crops improvement.

The set up objectives of the lab:

  1. Using molecular markers for varieties identification.

  2. Studying the genetic diversity for plant genetic resources.

  3. Introduce agricultural services for companies and farmers.

  4. Promote cooperation between NCARE and Universities.

  5. Detection of genetically modified crops.

Our strategy:

  1. Attracting trainees, scientists and those interested in our progress in the field of DNA fingerprinting.

  2. Public awareness is apriority for local communities to have a broad vision about biotechnology uses and risks. 

  3. Encourage commercialization products rise from biotechnology applications.

Molecular Techniques used at lab:

  1. Randomly Amplified Polymorphic DNA (RAPD)

  2. Simple Sequence Repeats (SSR)

  3. Inter-Simple Sequence Repeats (ISSR)

Researchers analysis form ( نموذج تحليل العينات الخاص بالباحثين )

Farmers and companies analysis form ( نموذج تحليل العينات الخاص بالمزارعين والشركات )

  

Laboratory and Quality Control Directorate achievements:

  1. Receiving numerous official delegations and university and school students which visited laboratories.

  2. Training many agronomists, engineers and Jordanian universities students in the laboratories.

  3. The laboratories received (7436) different samples and (30910) analyses were done. The estimated fees were (217365.25) JD,during 2013.

  4. The plant and fertilizer analysis laboratory was awarded a national accreditation in accordance to ISO/ICE 17025 requirements.